rnascope h202 protease reagents  (Advanced Cell Diagnostics Inc)

Journal: Cell reports

Article Title: Generation of human cerebral organoids with a structured outer subventricular zone

doi: 10.1016/j.celrep.2024.114031

Figure Lengend Snippet: (A) scRNA-seq Seurat clusters in control and LIF-treated organoids on day 60. The progenitor cluster 0 from control cortical organoids and the oRG cluster 2 from LIF-treated cortical organoids are highlighted. (B) Genes from analysis in (A) to mark selected populations of interest (neuronal cells, neuronal progenitors, astrocytes, and progenitor cells, including oRG) in control and LIF-treated organoids. (C) Enrichment analysis showing upregulation of STAT3 (left) and mTORC1 signaling (right) pathways in LIF-enriched cluster 2 compared to control cluster 0. (D) Gene Ontology (GO) Biological Process terms enriched in the LIF-specific cluster 2 compared to control cluster 0. (E) Migration-related genes upregulated in the LIF-specific cluster 2 compared to control cluster 0. (F) scRNA-seq signature enriched in LIF-treated organoids at day 60 (cluster 2) compared to control organoids (cluster 0) and mapped to fetal brain populations. The transcriptional profile of hPSC-derived oRG cells is enriched in fetal oRG compared to other radial glia populations and fetal astrocytes. (G) RNAscope for the LIFR , HOPX , and CDC42 transcripts, combined with a GFAP staining in control and LIF-treated WA09 cortical organoids at day 60. Nuclei are stained with DAPI and depicted either in blue. Scale bars, 50 μm.

Article Snippet: RNAscope H202 and Protease Reagents , ACD Bio , Cat#322381.

Techniques: Control, Migration, Derivative Assay, RNAscope, Staining

Journal: Cell reports

Article Title: Generation of human cerebral organoids with a structured outer subventricular zone

doi: 10.1016/j.celrep.2024.114031

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: RNAscope H202 and Protease Reagents , ACD Bio , Cat#322381.

Techniques: Recombinant, Purification, Marker, Virus, Membrane, Knock-Out, Imaging, Blocking Assay, Plasmid Preparation, RNAscope, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Multiplex Assay, RNA Sequencing Assay, Comparison, Software

Journal: eLife

Article Title: Identification of bipotent progenitors that give rise to myogenic and connective tissues in mouse

doi: 10.7554/eLife.70235

Figure Lengend Snippet: ( A–D ) scRNAseq analysis of the Myf5 Cre/+ ; Rosa26 mTmG/+ E11.5 dataset (2 datasets of 2 embryos were aggregated to generate this data, see Materials and methods). ( A ) UMAPs of Myf5 Cre/+ ; Rosa26 mTmG/+ E11.5 RNA velocity trajectories. ( B ) Expression plots of Alx4 and Pax3 , highlighting EOM and Anterior somite clusters, respectively. ( C ) Plots of Myogenic and Non-myogenic signatures, Coexpression score and heatmaps of top markers, highlighting the transition population in EOM and anterior somites. Cells are ordered based on their non-myogenic signature score (increasing). The coexpression score is the product of the myogenic and non-myogenic signatures. Cells presenting a coexpression score higher than 0.20 are highlighted in yellow. These cells represent the transition between the myogenic and non-myogenic fates. ( D ) UMAP of the EOM subset revealing the bipartite fate of Myf5 -expressing cells. ( E-G’ ) RNAscope on Myf5 Cre/+ ; Rosa26 mTmG/+ E14.5 tissue sections with Pdgfra (cyan) and Pdgfa (red) probes ( E-E’’ ), Bmprb1 (red) and Bmp4 (cyan) probes ( F-F’ ) and Ephb1 (red) and Efnb1 (cyan) probes ( G-G’ ). Myf5 -derived cells are labelled by membrane GFP staining (n = 3 embryos each). Red and yellow arrowheads indicate Myf5 -derived myogenic and non-myogenic cells respectively. The dotted lines highlight the boundary of the muscle masses. ( H ) Quantification of the Ligand-Receptor scores for each pair (see Materials and methods). Note that these ratios are negative in the case of Bmp and Eph (signaling from non-myogenic to myogenic) but positive for Pdgf (signaling from myogenic to non-myogenic). ( G ) Model of myogenic and non-myogenic cell communication following bifurcation from a bipotent cell. Figure 4—source data 1. Excel table summarizing the quantification displayed on .

Article Snippet: Commercial assay, kit , RNAscope H202 & Protease Plus Reagents , ACD/Bio-techne , Cat #: 322330 , .

Techniques: Expressing, Derivative Assay, Membrane, Staining

Journal: eLife

Article Title: Identification of bipotent progenitors that give rise to myogenic and connective tissues in mouse

doi: 10.7554/eLife.70235

Figure Lengend Snippet: ( A ) UMAP of Myf5 Cre/+ ; Rosa26 mTmG/+ E11.5 EOM dataset illustrating velocity confidence and velocity length. Higher confidence is found on both ends of the EOM cluster. ( B ) Expression pattern of Scube1 in the EOM subset (mostly in the non-myogenic compartment). ( C-C’’ ) Combined RNAscope for Scube1 and Myod/Myog/GFP immunostaining on transverse sections of Myf5 Cre/+ ; Rosa26 mTmG/+ at E14.5. ( D ) Model of the EOM muscle vs EOM origin compartmentalization used for quantification in (E). ( E ) Quantification of Scube1 signal in each compartment (n = 2 embryos). Figure 5—figure supplement 1—source data 1. Excel table summarizing the quantification displayed on .

Article Snippet: Commercial assay, kit , RNAscope H202 & Protease Plus Reagents , ACD/Bio-techne , Cat #: 322330 , .

Techniques: Expressing, Immunostaining